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21-deoxycortisol (21df  (Millipore)

 
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    Millipore 21-deoxycortisol (21df
    Preparation of a 15 steroid mix solution of steroids.
    21 Deoxycortisol (21df, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/21-deoxycortisol (21df/product/Millipore
    Average 90 stars, based on 1 article reviews
    21-deoxycortisol (21df - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry"

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    Journal: MethodsX

    doi: 10.1016/j.mex.2024.102728

    Preparation of a 15 steroid mix solution of steroids.
    Figure Legend Snippet: Preparation of a 15 steroid mix solution of steroids.

    Techniques Used: Concentration Assay

    Working internal standard (WIS) solution preparation in 2 mL or 10 mL batches.
    Figure Legend Snippet: Working internal standard (WIS) solution preparation in 2 mL or 10 mL batches.

    Techniques Used: Concentration Assay

    Calibration standard table detailing volume of calibration standard stock solution ( <xref ref-type= Table 2.2 ) and Working IS solution ( Table 2.3 ) to be added to each standard." title="Calibration standard table detailing volume of calibration standard stock solution (Table 2.2) and ... " property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Calibration standard table detailing volume of calibration standard stock solution ( Table 2.2 ) and Working IS solution ( Table 2.3 ) to be added to each standard.

    Techniques Used:

    Positive Multiple Reaction Monitoring parameters and retention times for each steroid and isotopically labelled internal standard as analysed on a QTrap 6500+. DP - Declustering Potential; CE - Collision Energy; CXP - Collision Cell Exit Potential. Quantifier (1) and qualifier (2) indicated accordingly. RT according to separation on a Kinetex C18 (150 × 2.1 mm; 2.6 µm), 0.3 mL/min methanol/water system. Assigned internal standard for each steroid indicated in final column.
    Figure Legend Snippet: Positive Multiple Reaction Monitoring parameters and retention times for each steroid and isotopically labelled internal standard as analysed on a QTrap 6500+. DP - Declustering Potential; CE - Collision Energy; CXP - Collision Cell Exit Potential. Quantifier (1) and qualifier (2) indicated accordingly. RT according to separation on a Kinetex C18 (150 × 2.1 mm; 2.6 µm), 0.3 mL/min methanol/water system. Assigned internal standard for each steroid indicated in final column.

    Techniques Used:

    Recovery of steroids from gold serum at three concentrations, by automated supported liquid extraction, following dilution with 0.1 % formic acid in water and elution with dichloromethane/isopropanol.
    Figure Legend Snippet: Recovery of steroids from gold serum at three concentrations, by automated supported liquid extraction, following dilution with 0.1 % formic acid in water and elution with dichloromethane/isopropanol.

    Techniques Used: Extraction

    Inter-assay accuracy (%RME) and precision (%RSD) for each steroid following automated supported liquid extraction and LC-MS/MS analysis.
    Figure Legend Snippet: Inter-assay accuracy (%RME) and precision (%RSD) for each steroid following automated supported liquid extraction and LC-MS/MS analysis.

    Techniques Used: Inter Assay, Extraction

    Inter-assay accuracy and precision ( n = 6) of quality control serum at low, medium and high levels, defined in the table,for each QC serum panel following automated supported liquid extraction and LC-MS/MS analysis.
    Figure Legend Snippet: Inter-assay accuracy and precision ( n = 6) of quality control serum at low, medium and high levels, defined in the table,for each QC serum panel following automated supported liquid extraction and LC-MS/MS analysis.

    Techniques Used: Inter Assay, Control, Extraction



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    Image Search Results


    Preparation of a 15 steroid mix solution of steroids.

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Preparation of a 15 steroid mix solution of steroids.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques: Concentration Assay

    Working internal standard (WIS) solution preparation in 2 mL or 10 mL batches.

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Working internal standard (WIS) solution preparation in 2 mL or 10 mL batches.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques: Concentration Assay

    Calibration standard table detailing volume of calibration standard stock solution ( <xref ref-type= Table 2.2 ) and Working IS solution ( Table 2.3 ) to be added to each standard." width="100%" height="100%">

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Calibration standard table detailing volume of calibration standard stock solution ( Table 2.2 ) and Working IS solution ( Table 2.3 ) to be added to each standard.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques:

    Positive Multiple Reaction Monitoring parameters and retention times for each steroid and isotopically labelled internal standard as analysed on a QTrap 6500+. DP - Declustering Potential; CE - Collision Energy; CXP - Collision Cell Exit Potential. Quantifier (1) and qualifier (2) indicated accordingly. RT according to separation on a Kinetex C18 (150 × 2.1 mm; 2.6 µm), 0.3 mL/min methanol/water system. Assigned internal standard for each steroid indicated in final column.

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Positive Multiple Reaction Monitoring parameters and retention times for each steroid and isotopically labelled internal standard as analysed on a QTrap 6500+. DP - Declustering Potential; CE - Collision Energy; CXP - Collision Cell Exit Potential. Quantifier (1) and qualifier (2) indicated accordingly. RT according to separation on a Kinetex C18 (150 × 2.1 mm; 2.6 µm), 0.3 mL/min methanol/water system. Assigned internal standard for each steroid indicated in final column.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques:

    Recovery of steroids from gold serum at three concentrations, by automated supported liquid extraction, following dilution with 0.1 % formic acid in water and elution with dichloromethane/isopropanol.

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Recovery of steroids from gold serum at three concentrations, by automated supported liquid extraction, following dilution with 0.1 % formic acid in water and elution with dichloromethane/isopropanol.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques: Extraction

    Inter-assay accuracy (%RME) and precision (%RSD) for each steroid following automated supported liquid extraction and LC-MS/MS analysis.

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Inter-assay accuracy (%RME) and precision (%RSD) for each steroid following automated supported liquid extraction and LC-MS/MS analysis.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques: Inter Assay, Extraction

    Inter-assay accuracy and precision ( n = 6) of quality control serum at low, medium and high levels, defined in the table,for each QC serum panel following automated supported liquid extraction and LC-MS/MS analysis.

    Journal: MethodsX

    Article Title: A practical approach to supported liquid extraction and measurement of 18 steroids in plasma and serum by targeted liquid chromatography tandem mass spectrometry

    doi: 10.1016/j.mex.2024.102728

    Figure Lengend Snippet: Inter-assay accuracy and precision ( n = 6) of quality control serum at low, medium and high levels, defined in the table,for each QC serum panel following automated supported liquid extraction and LC-MS/MS analysis.

    Article Snippet: All reagents used were of analytical grade or better ( Resource availability section ) cortisol (F), cortisone (E), corticosterone (B), 11-deoxycortisol (S), 21-deoxycortisol (21DF), 11-deoxycorticosterone (11DOC), testosterone (T), androstenedione (A4), 5α-dihydrotestosterone (DHT), dehydroepiandrosterone (DHEA), progesterone (P4), pregnenolone (P5), 17-hydroxypregnenolone (17OHP5), 17-hydroxyprogesterone (17OHP4), aldosterone (Aldo), estrone (E1), estradiol (E2), estriol (E3) and DC Mass Spect Gold Serum (MSG4000) were all purchased from Sigma-Aldrich and Cerilliant.

    Techniques: Inter Assay, Control, Extraction